BLASTX 1.4.6MP [16-Oct-94] [Build 06:35:34 Oct 18 1994] Reference: Gish, Warren and David J. States (1993). Identification of protein coding regions by database similarity search. Nat. Genet. 3:266-72. Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers, and David J. Lipman (1990). Basic local alignment search tool. J. Mol. Biol. 215:403-10. Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames. Query= gnl|dbest|62578 cDNA Human epidermal keratinocyte (406 letters) Translating both strands of query sequence in all 6 reading frames Database: Non-redundant PDB+SwissProt+SPupdate+PIR+GenPept+GPupdate 130,171 sequences; 37,236,619 total letters. Searching..................................................done Smallest Sum Reading High Probability Sequences producing High-scoring Segment Pairs: Frame Score P(N) N pir|A54105|A54105 fibrillin-2 precursor - human -3 41 1.3e-05 4 gp|X74760|MMNOTC_1 Notch 3 [Mus musculus] -3 46 1.6e-05 4 gp|U03272|HSU03272_1 fibrillin-2 [Homo sapiens] -2 39 0.00022 4 sp|P33479|IE18_PRVKA IMMEDIATE-EARLY PROTEIN IE180. >p... +2 41 0.025 3 sp|P11675|IE18_PRVIF IMMEDIATE-EARLY PROTEIN IE180. >g... +2 41 0.026 3 gp|X51985|HSLAG3_3 Human LAG-3 mRNA for CD4-related ... -3 39 0.028 3 sp|P18627|LAG3_HUMAN LYMPHOCYTE ACTIVATION GENE-3 PROT... -3 39 0.032 3 pir|S18252|S18252 perlecan - mouse >gp|M77174|MUSPE... +1 41 0.59 3 gp|M64780|RATAGR_1 agrin [Rattus norvegicus] -2 32 0.75 4 gp|M64780|RATAGR_2 agrin [Rattus norvegicus] -2 32 0.76 4 sp|P25304|AGRI_RAT AGRIN PRECURSOR. >pir|JH0399|AGRT... -2 32 0.76 4 pir|B47301|B47301 VirB4 homolog - Bordetella pertussis -3 39 0.85 3 pir|JC1103|JC1103 lens fiber cell beaded-filament p... -2 40 0.95 3 gp|D10866|RATCP94_1 lens fiber cell beaded-filament s... -2 40 0.95 3 sp|P10079|EGFH_STRPU EPIDERMAL GROWTH FACTOR HOMOLOG (... -2 37 0.999 3 gp|L24521|HUMTRRP_1 transformation-related protein [H... -2 54 0.999 2 pir|S12643|S12643 regulatory protein trpI - Pseudom... +3 49 0.999 2 pir|A44467|A44467 voltage-dependent calcium channel... -3 38 0.9995 3 sp|P23962|ALUD_HUMAN !!!! ALU CLASS D WARNING ENTRY !!... +3 45 0.99994 2 >pir|A54105|A54105 fibrillin-2 precursor - human Length = 2918 Minus Strand HSPs: Score = 41 (18.9 bits), Expect = 0.021, Sum P(3) = 0.020 Identities = 7/25 (28%), Positives = 9/25 (36%), Frame = -3 Query: 278 TLAIEPAYAHDHQTCLRPCPCGPHP 204 T P + H C+ CG P Sbjct: 2554 TCKCPPGFTQHHTACIDNNECGSQP 2578 Score = 39 (17.9 bits), Expect = 1.3e-05, Sum P(4) = 1.3e-05 Identities = 8/26 (30%), Positives = 11/26 (42%), Frame = -2 Query: 234 LTPMSMWTPPQGQPLWSSRHCRTETG 157 + P M P G+ CRT+ G Sbjct: 2321 ICPPGMARRPDGEGCVDENECRTKPG 2346 Score = 38 (17.5 bits), Expect = 1.3e-05, Sum P(4) = 1.3e-05 Identities = 7/12 (58%), Positives = 7/12 (58%), Frame = -3 Query: 392 SCRPGWSAMGDK 357 SCR GW G K Sbjct: 1393 SCREGWIGNGIK 1404 Score = 37 (17.0 bits), Expect = 1.3e-05, Sum P(4) = 1.3e-05 Identities = 5/8 (62%), Positives = 7/8 (87%), Frame = -2 Query: 273 GHRTCLCP 250 G+ TC+CP Sbjct: 1712 GNYTCICP 1719 Score = 37 (17.0 bits), Expect = 1.7e-05, Sum P(4) = 1.7e-05 Identities = 6/12 (50%), Positives = 8/12 (66%), Frame = -3 Query: 392 SCRPGWSAMGDK 357 SC PG+ A D+ Sbjct: 1225 SCNPGYQATPDR 1236 Score = 36 (16.6 bits), Expect = 1.3e-05, Sum P(4) = 1.3e-05 Identities = 6/9 (66%), Positives = 8/9 (88%), Frame = -1 Query: 43 DQHLGKVGE 17 D HLG++GE Sbjct: 2892 DYHLGELGE 2900 Score = 34 (15.6 bits), Expect = 5.4e-05, Sum P(4) = 5.4e-05 Identities = 5/8 (62%), Positives = 6/8 (75%), Frame = -2 Query: 84 NQPKSPCP 61 N+P PCP Sbjct: 1772 NKPCEPCP 1779 Score = 32 (14.7 bits), Expect = 7.2e-05, Sum P(4) = 7.2e-05 Identities = 4/8 (50%), Positives = 5/8 (62%), Frame = -3 Query: 389 CRPGWSAM 366 C PGW + Sbjct: 97 CCPGWKTL 104 Score = 31 (14.3 bits), Expect = 0.00022, Sum P(4) = 0.00022 Identities = 7/18 (38%), Positives = 8/18 (44%), Frame = -1 Query: 58 PGAVTDQHLGKVGEWRVC 5 PG D H GK + C Sbjct: 1794 PGFTFDIHTGKAVDIDEC 1811 Score = 31 (14.3 bits), Expect = 0.0016, Sum P(4) = 0.0016 Identities = 5/13 (38%), Positives = 7/13 (53%), Frame = -3 Query: 224 CPCGPHPRASLSG 186 C C P + S +G Sbjct: 874 CECSPGSKLSSTG 886 >gp|X74760|MMNOTC_1 Notch 3 [Mus musculus] Length = 2318 Minus Strand HSPs: Score = 46 (21.2 bits), Expect = 1.4, Sum P(3) = 0.75 Identities = 8/19 (42%), Positives = 8/19 (42%), Frame = -3 Query: 263 PAYAHDHQTCLRPCPCGPH 207 P D C PCGPH Sbjct: 806 PRCQQDVDECAGASPCGPH 824 Score = 37 (17.0 bits), Expect = 1.4, Sum P(3) = 0.75 Identities = 5/6 (83%), Positives = 5/6 (83%), Frame = -3 Query: 389 CRPGWS 372 C PGWS Sbjct: 721 CEPGWS 726 Score = 35 (16.1 bits), Expect = 3.3, Sum P(3) = 0.96 Identities = 5/6 (83%), Positives = 5/6 (83%), Frame = -3 Query: 392 SCRPGW 375 SC PGW Sbjct: 798 SCPPGW 803 Score = 35 (16.1 bits), Expect = 1.7e+02, Sum P(3) = 1.0 Identities = 6/12 (50%), Positives = 8/12 (66%), Frame = -2 Query: 234 LTPMSMWTPPQG 199 + P + TPPQG Sbjct: 1763 VAPATALTPPQG 1774 Score = 32 (14.7 bits), Expect = 1.6e-05, Sum P(4) = 1.6e-05 Identities = 4/12 (33%), Positives = 9/12 (75%), Frame = -1 Query: 76 QVSMPCPGAVTD 41 ++++ CPG + D Sbjct: 2084 KLTLACPGPLAD 2095 Score = 32 (14.7 bits), Expect = 0.70, Sum P(4) = 0.51 Identities = 5/9 (55%), Positives = 5/9 (55%), Frame = -2 Query: 276 SGHRTCLCP 250 SG C CP Sbjct: 373 SGRAICTCP 381 Score = 32 (14.7 bits), Expect = 1.4, Sum P(3) = 0.75 Identities = 7/15 (46%), Positives = 7/15 (46%), Frame = -2 Query: 45 RTSTWARLESGGCVL 1 R R GGCVL Sbjct: 2144 RAGPLGRQPPGGCVL 2158 Score = 31 (14.3 bits), Expect = 17., Sum P(3) = 1.0 Identities = 4/5 (80%), Positives = 4/5 (80%), Frame = -3 Query: 389 CRPGW 375 C PGW Sbjct: 68 CLPGW 72 >gp|U03272|HSU03272_1 fibrillin-2 [Homo sapiens] Length = 2911 Minus Strand HSPs: Score = 39 (17.9 bits), Expect = 6.4, Sum P(3) = 1.0 Identities = 8/26 (30%), Positives = 11/26 (42%), Frame = -2 Query: 234 LTPMSMWTPPQGQPLWSSRHCRTETG 157 + P M P G+ CRT+ G Sbjct: 2321 ICPPGMARRPDGEGCVDENECRTKPG 2346 Score = 38 (17.5 bits), Expect = 6.4, Sum P(3) = 1.0 Identities = 7/12 (58%), Positives = 7/12 (58%), Frame = -3 Query: 392 SCRPGWSAMGDK 357 SCR GW G K Sbjct: 1393 SCREGWIGNGIK 1404 Score = 37 (17.0 bits), Expect = 6.4, Sum P(3) = 1.0 Identities = 5/8 (62%), Positives = 7/8 (87%), Frame = -2 Query: 273 GHRTCLCP 250 G+ TC+CP Sbjct: 1712 GNYTCICP 1719 Score = 37 (17.0 bits), Expect = 9.4, Sum P(3) = 1.0 Identities = 6/12 (50%), Positives = 8/12 (66%), Frame = -3 Query: 392 SCRPGWSAMGDK 357 SC PG+ A D+ Sbjct: 1225 SCNPGYQATPDR 1236 Score = 34 (15.6 bits), Expect = 0.00022, Sum P(4) = 0.00022 Identities = 5/8 (62%), Positives = 6/8 (75%), Frame = -2 Query: 84 NQPKSPCP 61 N+P PCP Sbjct: 1772 NKPCEPCP 1779 Score = 32 (14.7 bits), Expect = 61., Sum P(3) = 1.0 Identities = 4/8 (50%), Positives = 5/8 (62%), Frame = -3 Query: 389 CRPGWSAM 366 C PGW + Sbjct: 97 CCPGWKTL 104 Score = 31 (14.3 bits), Expect = 0.00022, Sum P(4) = 0.00022 Identities = 7/18 (38%), Positives = 8/18 (44%), Frame = -1 Query: 58 PGAVTDQHLGKVGEWRVC 5 PG D H GK + C Sbjct: 1794 PGFTFDIHTGKAVDIDEC 1811 Score = 31 (14.3 bits), Expect = 0.0066, Sum P(4) = 0.0065 Identities = 5/13 (38%), Positives = 7/13 (53%), Frame = -3 Query: 224 CPCGPHPRASLSG 186 C C P + S +G Sbjct: 874 CECSPGSKLSSTG 886 >sp|P33479|IE18_PRVKA IMMEDIATE-EARLY PROTEIN IE180. >pir|A45344|A45344 immediate-early protein - suid herpesvirus 1 (strain Kaplan) >gp|M34651|SH1PROIE_1 immediate-early protein [Pseudorabies virus] Length = 1446 Plus Strand HSPs: Score = 41 (18.9 bits), Expect = 0.025, Sum P(3) = 0.025 Identities = 7/8 (87%), Positives = 7/8 (87%), Frame = +2 Query: 236 RFDGHGHR 259 R DGHGHR Sbjct: 911 RGDGHGHR 918 Score = 38 (17.5 bits), Expect = 0.025, Sum P(3) = 0.025 Identities = 8/16 (50%), Positives = 9/16 (56%), Frame = +3 Query: 18 SPTLPKCWSVTAPGQG 65 SP P V+APG G Sbjct: 230 SPASPAAGPVSAPGGG 245 Score = 35 (16.1 bits), Expect = 0.025, Sum P(3) = 0.025 Identities = 5/5 (100%), Positives = 5/5 (100%), Frame = +1 Query: 214 PHGHG 228 PHGHG Sbjct: 584 PHGHG 588 >sp|P11675|IE18_PRVIF IMMEDIATE-EARLY PROTEIN IE180. >gp|X15120|HEPVIE_1 Pseudorabies virus immediate-early gene. [Pseudorabies virus] Length = 1461 Plus Strand HSPs: Score = 41 (18.9 bits), Expect = 0.026, Sum P(3) = 0.026 Identities = 7/8 (87%), Positives = 7/8 (87%), Frame = +2 Query: 236 RFDGHGHR 259 R DGHGHR Sbjct: 922 RGDGHGHR 929 Score = 38 (17.5 bits), Expect = 0.026, Sum P(3) = 0.026 Identities = 8/16 (50%), Positives = 9/16 (56%), Frame = +3 Query: 18 SPTLPKCWSVTAPGQG 65 SP P V+APG G Sbjct: 238 SPASPAAGPVSAPGGG 253 Score = 37 (17.0 bits), Expect = 0.50, Sum P(3) = 0.39 Identities = 8/19 (42%), Positives = 10/19 (52%), Frame = +2 Query: 233 SRFDGHGHRQVRWPEFFQL 289 +R D VRWPE +L Sbjct: 1193 ARLDPAAQCAVRWPECREL 1211 Score = 35 (16.1 bits), Expect = 0.026, Sum P(3) = 0.026 Identities = 5/5 (100%), Positives = 5/5 (100%), Frame = +1 Query: 214 PHGHG 228 PHGHG Sbjct: 595 PHGHG 599 >gp|X51985|HSLAG3_3 Human LAG-3 mRNA for CD4-related protein involved in lymphocyte activation. [Homo sapiens] Length = 470 Minus Strand HSPs: Score = 39 (17.9 bits), Expect = 0.028, Sum P(3) = 0.028 Identities = 8/16 (50%), Positives = 8/16 (50%), Frame = -3 Query: 227 PCPCGPHPRASLSGAP 180 P GPHP A S P Sbjct: 51 PLAPGPHPAAPSSWGP 66 Score = 39 (17.9 bits), Expect = 0.23, Sum P(3) = 0.21 Identities = 6/8 (75%), Positives = 6/8 (75%), Frame = -2 Query: 216 WTPPQGQP 193 WTPP G P Sbjct: 270 WTPPGGGP 277 Score = 37 (17.0 bits), Expect = 0.23, Sum P(3) = 0.21 Identities = 7/16 (43%), Positives = 11/16 (68%), Frame = -1 Query: 394 TLVAQAGVQWATKQDS 347 +L+ +AGV W + DS Sbjct: 26 SLLRRAGVTWQHQPDS 41 Score = 30 (13.8 bits), Expect = 0.23, Sum P(3) = 0.21 Identities = 8/16 (50%), Positives = 9/16 (56%), Frame = -1 Query: 79 AQVSMPCPGAVTDQHL 32 AQ S PGA+ HL Sbjct: 407 AQRSGRAPGALPAGHL 422 >sp|P18627|LAG3_HUMAN LYMPHOCYTE ACTIVATION GENE-3 PROTEIN PRECURSOR (LAG-3) (FDC PROTEIN). >pir|S11246|S11246 LAG-3 protein precursor - human >gp|X51985|HSLAG3_2 Human LAG-3 mRNA for CD4-related protein involved in lymphocyte activation. [Homo sapiens] Length = 498 Minus Strand HSPs: Score = 39 (17.9 bits), Expect = 0.032, Sum P(3) = 0.032 Identities = 8/16 (50%), Positives = 8/16 (50%), Frame = -3 Query: 227 PCPCGPHPRASLSGAP 180 P GPHP A S P Sbjct: 79 PLAPGPHPAAPSSWGP 94 Score = 39 (17.9 bits), Expect = 0.26, Sum P(3) = 0.23 Identities = 6/8 (75%), Positives = 6/8 (75%), Frame = -2 Query: 216 WTPPQGQP 193 WTPP G P Sbjct: 298 WTPPGGGP 305 Score = 37 (17.0 bits), Expect = 0.26, Sum P(3) = 0.23 Identities = 7/16 (43%), Positives = 11/16 (68%), Frame = -1 Query: 394 TLVAQAGVQWATKQDS 347 +L+ +AGV W + DS Sbjct: 54 SLLRRAGVTWQHQPDS 69 Score = 30 (13.8 bits), Expect = 0.26, Sum P(3) = 0.23 Identities = 8/16 (50%), Positives = 9/16 (56%), Frame = -1 Query: 79 AQVSMPCPGAVTDQHL 32 AQ S PGA+ HL Sbjct: 435 AQRSGRAPGALPAGHL 450 >pir|S18252|S18252 perlecan - mouse >gp|M77174|MUSPERPA_1 perlecan [Mus musculus] Length = 3707 Plus Strand HSPs: Score = 41 (18.9 bits), Expect = 0.88, Sum P(3) = 0.59 Identities = 7/12 (58%), Positives = 7/12 (58%), Frame = +1 Query: 7 TPSTLQPCPSAG 42 TP QPCP G Sbjct: 1202 TPQDCQPCPCYG 1213 Score = 40 (18.4 bits), Expect = 0.88, Sum P(3) = 0.59 Identities = 6/9 (66%), Positives = 6/9 (66%), Frame = +2 Query: 245 GHGHRQVRW 271 GH H QV W Sbjct: 2077 GHAHAQVTW 2085 Score = 36 (16.6 bits), Expect = 0.88, Sum P(3) = 0.59 Identities = 7/12 (58%), Positives = 7/12 (58%), Frame = +1 Query: 187 PERLALGWGPHG 222 PE L G PHG Sbjct: 1270 PEVLGCGCDPHG 1281 >gp|M64780|RATAGR_1 agrin [Rattus norvegicus] Length = 1937 Minus Strand HSPs: Score = 32 (14.7 bits), Expect = 1.8e+03, Sum P(3) = 1.0 Identities = 4/11 (36%), Positives = 7/11 (63%), Frame = -2 Query: 282 KNSGHRTCLCP 250 + +G C+CP Sbjct: 806 EKAGFAQCICP 816 Score = 32 (14.7 bits), Expect = 9.9e+03, Sum P(2) = 1.0 Identities = 7/13 (53%), Positives = 7/13 (53%), Frame = -2 Query: 57 LVP*RTSTWARLE 19 LVP W RLE Sbjct: 1334 LVPVEPGRWHRLE 1346 Score = 31 (14.3 bits), Expect = 1.4, Sum P(4) = 0.75 Identities = 5/12 (41%), Positives = 6/12 (50%), Frame = -3 Query: 227 PCPCGPHPRASL 192 P P PH S+ Sbjct: 908 PLPISPHTTVSI 919 Score = 31 (14.3 bits), Expect = 1.8e+03, Sum P(3) = 1.0 Identities = 5/5 (100%), Positives = 5/5 (100%), Frame = -3 Query: 392 SCRPG 378 SCRPG Sbjct: 759 SCRPG 763 >gp|M64780|RATAGR_2 agrin [Rattus norvegicus] Length = 1940 Minus Strand HSPs: Score = 32 (14.7 bits), Expect = 1.8e+03, Sum P(3) = 1.0 Identities = 4/11 (36%), Positives = 7/11 (63%), Frame = -2 Query: 282 KNSGHRTCLCP 250 + +G C+CP Sbjct: 809 EKAGFAQCICP 819 Score = 32 (14.7 bits), Expect = 9.9e+03, Sum P(2) = 1.0 Identities = 7/13 (53%), Positives = 7/13 (53%), Frame = -2 Query: 57 LVP*RTSTWARLE 19 LVP W RLE Sbjct: 1337 LVPVEPGRWHRLE 1349 Score = 31 (14.3 bits), Expect = 1.4, Sum P(4) = 0.76 Identities = 5/12 (41%), Positives = 6/12 (50%), Frame = -3 Query: 227 PCPCGPHPRASL 192 P P PH S+ Sbjct: 911 PLPISPHTTVSI 922 Score = 31 (14.3 bits), Expect = 1.8e+03, Sum P(3) = 1.0 Identities = 5/5 (100%), Positives = 5/5 (100%), Frame = -3 Query: 392 SCRPG 378 SCRPG Sbjct: 762 SCRPG 766 >sp|P25304|AGRI_RAT AGRIN PRECURSOR. >pir|JH0399|AGRT agrin - rat Length = 1959 Minus Strand HSPs: Score = 32 (14.7 bits), Expect = 1.8e+03, Sum P(3) = 1.0 Identities = 4/11 (36%), Positives = 7/11 (63%), Frame = -2 Query: 282 KNSGHRTCLCP 250 + +G C+CP Sbjct: 809 EKAGFAQCICP 819 Score = 32 (14.7 bits), Expect = 1.0e+04, Sum P(2) = 1.0 Identities = 7/13 (53%), Positives = 7/13 (53%), Frame = -2 Query: 57 LVP*RTSTWARLE 19 LVP W RLE Sbjct: 1337 LVPVEPGRWHRLE 1349 Score = 31 (14.3 bits), Expect = 1.4, Sum P(4) = 0.76 Identities = 5/12 (41%), Positives = 6/12 (50%), Frame = -3 Query: 227 PCPCGPHPRASL 192 P P PH S+ Sbjct: 911 PLPISPHTTVSI 922 Score = 31 (14.3 bits), Expect = 1.8e+03, Sum P(3) = 1.0 Identities = 5/5 (100%), Positives = 5/5 (100%), Frame = -3 Query: 392 SCRPG 378 SCRPG Sbjct: 762 SCRPG 766 >pir|B47301|B47301 VirB4 homolog - Bordetella pertussis Length = 824 Minus Strand HSPs: Score = 39 (17.9 bits), Expect = 1.9, Sum P(3) = 0.85 Identities = 7/11 (63%), Positives = 7/11 (63%), Frame = -3 Query: 56 WCRDGPALGQG 24 WCRD A G G Sbjct: 596 WCRDDGAGGTG 606 Score = 37 (17.0 bits), Expect = 1.9, Sum P(3) = 0.85 Identities = 6/17 (35%), Positives = 11/17 (64%), Frame = -2 Query: 225 MSMWTPPQGQPLWSSRH 175 +S+ + P GQP + + H Sbjct: 419 LSLLSTPSGQPFYFNFH 435 Score = 34 (15.6 bits), Expect = 1.9, Sum P(3) = 0.85 Identities = 6/11 (54%), Positives = 9/11 (81%), Frame = -3 Query: 386 RPGWSAMGDKA 354 RPG +A+G +A Sbjct: 132 RPGHAALGKRA 142 >pir|JC1103|JC1103 lens fiber cell beaded-filament protein CP94 - rat (fragment) Length = 533 Minus Strand HSPs: Score = 40 (18.4 bits), Expect = 3.0, Sum P(3) = 0.95 Identities = 5/9 (55%), Positives = 8/9 (88%), Frame = -2 Query: 75 KSPCPVLVP 49 + PCPV++P Sbjct: 482 RRPCPVIIP 490 Score = 35 (16.1 bits), Expect = 3.0, Sum P(3) = 0.95 Identities = 7/14 (50%), Positives = 9/14 (64%), Frame = -2 Query: 231 TPMSMWTPPQGQPL 190 TP+S+ TP G L Sbjct: 237 TPISLITPSHGASL 250 Score = 33 (15.2 bits), Expect = 3.0, Sum P(3) = 0.95 Identities = 6/14 (42%), Positives = 9/14 (64%), Frame = -3 Query: 281 RTLAIEPAYAHDHQ 240 +T A+E A H H+ Sbjct: 155 QTTALEQAIKHAHE 168 >gp|D10866|RATCP94_1 lens fiber cell beaded-filament structure protein [Rattus norvegicus] Length = 533 Minus Strand HSPs: Score = 40 (18.4 bits), Expect = 3.0, Sum P(3) = 0.95 Identities = 5/9 (55%), Positives = 8/9 (88%), Frame = -2 Query: 75 KSPCPVLVP 49 + PCPV++P Sbjct: 482 RRPCPVIIP 490 Score = 35 (16.1 bits), Expect = 3.0, Sum P(3) = 0.95 Identities = 7/14 (50%), Positives = 9/14 (64%), Frame = -2 Query: 231 TPMSMWTPPQGQPL 190 TP+S+ TP G L Sbjct: 237 TPISLITPSHGASL 250 Score = 33 (15.2 bits), Expect = 3.0, Sum P(3) = 0.95 Identities = 6/14 (42%), Positives = 9/14 (64%), Frame = -3 Query: 281 RTLAIEPAYAHDHQ 240 +T A+E A H H+ Sbjct: 155 QTTALEQAIKHAHE 168 >sp|P10079|EGFH_STRPU EPIDERMAL GROWTH FACTOR HOMOLOG (UEGF-1) (FRAGMENT). >pir|A29316|A29316 epidermal growth factor homolog - sea urchin (Strongylocentrotus purpuratus) (fragment) >gp|M17421|SUSGFE_1 S.purpuratus epidermal growth factor homolog mRNA, 3' end. [Strongylocentrotus purpuratus] Length = 482 Minus Strand HSPs: Score = 37 (17.0 bits), Expect = 6.7, Sum P(3) = 1.0 Identities = 6/15 (40%), Positives = 9/15 (60%), Frame = -2 Query: 198 QPLWSSRHCRTETGE 154 QP ++ HC T+ E Sbjct: 115 QPGYTGTHCETDIDE 129 Score = 33 (15.2 bits), Expect = 6.7, Sum P(3) = 1.0 Identities = 4/6 (66%), Positives = 5/6 (83%), Frame = -3 Query: 389 CRPGWS 372 C PGW+ Sbjct: 76 CAPGWT 81 Score = 32 (14.7 bits), Expect = 6.7, Sum P(3) = 1.0 Identities = 6/14 (42%), Positives = 8/14 (57%), Frame = -1 Query: 70 SMPCPGAVTDQHLG 29 SMPC T ++G Sbjct: 284 SMPCQNGATCVNVG 297 >gp|L24521|HUMTRRP_1 transformation-related protein [Homo sapiens] Length = 368 Minus Strand HSPs: Score = 54 (24.8 bits), Expect = 6.7, Sum P(2) = 1.0 Identities = 10/10 (100%), Positives = 10/10 (100%), Frame = -2 Query: 393 LLSPRLECNG 364 LLSPRLECNG Sbjct: 5 LLSPRLECNG 14 Score = 33 (15.2 bits), Expect = 6.7, Sum P(2) = 1.0 Identities = 6/15 (40%), Positives = 9/15 (60%), Frame = -2 Query: 246 PSNLLTPMSMWTPPQ 202 P + L P ++ PPQ Sbjct: 323 PVSFLNPHTLTWPPQ 337 >pir|S12643|S12643 regulatory protein trpI - Pseudomonas aeruginosa >gp|X51868|PATRPIG_1 TrpI protein [Pseudomonas aeruginosa] Length = 294 Plus Strand HSPs: Score = 49 (22.5 bits), Expect = 6.8, Sum P(2) = 1.0 Identities = 8/19 (42%), Positives = 12/19 (63%), Frame = +3 Query: 12 LHSPTLPKCWSVTAPGQGM 68 LH+ + P+ W A GQG+ Sbjct: 194 LHTASRPQAWPAWAAGQGL 212 Score = 33 (15.2 bits), Expect = 6.8, Sum P(2) = 1.0 Identities = 5/10 (50%), Positives = 6/10 (60%), Frame = +2 Query: 194 GWPWGGVHMD 223 G PWG + D Sbjct: 256 GGPWGFIETD 265 >pir|A44467|A44467 voltage-dependent calcium channel complex alpha-1 chain mbC - mouse >gp|L01776|MUSDHPCC_1 L-type calcium channel alpha-1 [Mus musculus] Length = 2139 Minus Strand HSPs: Score = 38 (17.5 bits), Expect = 7.6, Sum P(3) = 1.0 Identities = 7/15 (46%), Positives = 8/15 (53%), Frame = -3 Query: 254 AHDHQTCLRPCPCGP 210 +H T RPCP P Sbjct: 1941 SHSPTTFPRPCPTPP 1955 Score = 36 (16.6 bits), Expect = 7.6, Sum P(3) = 1.0 Identities = 6/14 (42%), Positives = 8/14 (57%), Frame = -3 Query: 77 PSLHALSWCRDGPA 36 PS+H SW + A Sbjct: 1987 PSIHCSSWSEETTA 2000 Score = 35 (16.1 bits), Expect = 7.6, Sum P(3) = 1.0 Identities = 4/5 (80%), Positives = 5/5 (100%), Frame = -3 Query: 389 CRPGW 375 C+PGW Sbjct: 332 CKPGW 336 >sp|P23962|ALUD_HUMAN !!!! ALU CLASS D WARNING ENTRY !!!! >pir|D40201|D40201 artifact-warning sequence (translated ALU class D) - human Length = 579 Plus Strand HSPs: Score = 45 (20.7 bits), Expect = 9.8, Sum P(2) = 1.0 Identities = 7/7 (100%), Positives = 7/7 (100%), Frame = +3 Query: 369 CTPAWAT 389 CTPAWAT Sbjct: 275 CTPAWAT 281 Score = 36 (16.6 bits), Expect = 9.8, Sum P(2) = 1.0 Identities = 7/14 (50%), Positives = 8/14 (57%), Frame = +3 Query: 21 PTLPKCWSVTAPGQ 62 P L + W V AP Q Sbjct: 107 PALXEPWQVYAPAQ 120 Parameters: -filter=seg+xnu V=500 B=250 -ctxfactor=5.18 E=10 Query ----- As Used ----- ----- Computed ---- Frame MatID Matrix name Lambda K H Lambda K H Std. 0 BLOSUM62 0.319 0.135 0.396 +3 0 BLOSUM62 0.319 0.135 0.396 0.340 0.141 0.540 +2 0 BLOSUM62 0.319 0.135 0.396 0.330 0.145 0.505 +1 0 BLOSUM62 0.319 0.135 0.396 0.333 0.140 0.518 -1 0 BLOSUM62 0.319 0.135 0.396 0.319 0.129 0.412 -2 0 BLOSUM62 0.319 0.135 0.396 0.338 0.144 0.523 -3 0 BLOSUM62 0.319 0.135 0.396 0.327 0.138 0.497 Query Frame MatID Length Eff.Length E S W T X E2 S2 +3 0 134 70 10. 54 3 12 22 0.12 30 +2 0 135 83 10. 55 3 12 22 0.11 31 +1 0 135 91 10. 56 3 12 22 0.090 32 -1 0 135 71 10. 54 3 12 22 0.12 30 -2 0 135 96 10. 56 3 12 22 0.096 32 -3 0 134 86 10. 56 3 12 22 0.12 31 Statistics: Query Expected Observed HSPs HSPs Frame MatID High Score High Score Reportable Reported +3 0 57 (26.2 bits) 52 (23.9 bits) 5 5 +2 0 59 (27.1 bits) 52 (23.9 bits) 5 5 +1 0 60 (27.6 bits) 52 (23.9 bits) 4 4 -1 0 58 (26.7 bits) 48 (22.1 bits) 9 9 -2 0 60 (27.6 bits) 57 (26.2 bits) 25 25 -3 0 59 (27.1 bits) 57 (26.2 bits) 29 29 Query Neighborhd Word Excluded Failed Successful Overlaps Frame MatID Words Hits Hits Extensions Extensions Excluded +3 0 1640 3283081 751048 2523911 8122 3 +2 0 1602 3875679 824313 3043115 8251 2 +1 0 2119 4623683 1027944 3589967 5772 3 -1 0 1479 2941300 612930 2323210 5160 1 -2 0 1956 4753133 1061303 3685646 6184 13 -3 0 2018 4704702 1052500 3638708 13494 52 Database: Non-redundant PDB+SwissProt+SPupdate+PIR+GenPept+GPupdate Release date: 7:09 PM EDT Oct 18, 1994 Posted date: 7:36 PM EDT Oct 18, 1994 # of letters in database: 37,236,619 # of sequences in database: 130,171 # of database sequences satisfying E: 19 No. of states in DFA: 583 (57 KB) Total size of DFA: 161 KB (192 KB) Time to generate neighborhood: 0.05u 0.01s 0.06t Real: 00:00:00 No. of processors used: 8 Time to search database: 159.15u 2.60s 161.75t Real: 00:00:21 Total cpu time: 159.29u 2.93s 162.22t Real: 00:00:21